Publication Type Journal Article
Title Biological activity and cellular uptake of [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complex
Authors Tânia S. Morais Filipa C. Santos Leonor Côrte-Real Fernanda M. Marques M Paula Robalo Paulo J. Amorim Madeira M. Helena Garcia
Groups BioMol BIOIN
Journal JOURNAL OF INORGANIC BIOCHEMISTRY
Year 2013
Month May
Volume 122
Number
Pages 8-17
Abstract Anticancer activity of the new [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] (Me(2)bpy = 4,4 -dimethyl-2,2 -bipyridine) complex was evaluated in vitro against several human cancer cell lines, namely A2780, A2780CisR, HT29, MCF7, MDAMB231 and PC3. Remarkably, the IC50 values, placed in the nanomolar and sub-micromolar range, largely exceeded the activity of cisplatin. Binding to human serum albumin, either HSA (human serum albumin) or HSA(faf) (fatty acid-free human serum albumin) does not affect the complex activity. Fluorescence studies revealed that the present ruthenium complex strongly quench the intrinsic fluorescence of albumin. Cell death by the [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complex was reduced in the presence of endocytosis modulators and at low temperature, suggesting an energy-dependent mechanism consistent with endocytosis. On the whole, the biological activity evaluated herein suggests that the complex could be a promising anticancer agent. (C) 2013 Elsevier Inc. All rights reserved.
DOI http://dx.doi.org/10.1016/j.jinorgbio.2013.01.011
ISBN
Publisher
Book Title
ISSN 0162-0134
EISSN
Conference Name
Bibtex ID ISI:000316832900002
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