| Publication Type |
Journal Article |
| Title |
Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death |
| Authors |
Sofia Nolasco J Bellido J Goncalves JC Zabala M. H. A. Soares |
| Groups |
|
| Journal |
FEBS LETTERS |
| Year |
2005 |
| Month |
July |
| Volume |
579 |
| Number |
17 |
| Pages |
3515-3524 |
| Abstract |
Microtubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| DOI |
http://dx.doi.org/10.1016/j.febslet.2005.05.022 |
| ISBN |
|
| Publisher |
|
| Book Title |
|
| ISSN |
0014-5793 |
| EISSN |
|
| Conference Name |
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| Bibtex ID |
ISI:000230335600009 |
| Observations |
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