| Publication Type |
Journal Article |
| Title |
Assessment of beta-carotene content, cell physiology and morphology of the yellow yeast Rhodotorula glutinis mutant 400A15 using flow cytometry |
| Authors |
Raffaela Cutzu Ana Clemente Alberto Reis Beatriz Nobre Ilaria Mannazzu Jose Roseiro Teresa Lopes da Silva |
| Groups |
MET |
| Journal |
JOURNAL OF INDUSTRIAL MICROBIOLOGY \& BIOTECHNOLOGY |
| Year |
2013 |
| Month |
August |
| Volume |
40 |
| Number |
8 |
| Pages |
865-875 |
| Abstract |
Flow cytometry was used to assess beta-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (k (L) a) and carbon to nitrogen ratios (C/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest beta-carotene content (0.79 mg g(-1) DCW) at the lowest k (L) a and C/N (5 x 10(-3) s(-1) and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60 g L-1. The highest ratio of cells with permeabilised membranes (2.6 \%), and the highest cell size and granularity were also obtained under these conditions. It was observed that C/N showed a stronger influence than the k (L) a on the measured cell parameters. |
| DOI |
http://dx.doi.org/10.1007/s10295-013-1278-2 |
| ISBN |
|
| Publisher |
|
| Book Title |
|
| ISSN |
1367-5435 |
| EISSN |
|
| Conference Name |
|
| Bibtex ID |
ISI:000321972400009 |
| Observations |
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