Publication Type Journal Article
Title Stimuli-Responsive magnetic nanoparticles for monoclonal antibody purification
Authors Luis Borlido Leila Moura Ana M. Azevedo Ana C. A. Roque M. R. Aires-Barros José Paulo Farinha
Groups
Journal BIOTECHNOLOGY JOURNAL
Year 2013
Month June
Volume 8
Number 6
Pages 709-717
Abstract Monoclonal antibodies (mAbs) are important therapeutic proteins. One of the challenges facing large-scale production of monoclonal antibodies is the capacity bottleneck in downstream processing, which can be circumvented by using magnetic stimuli-responsive polymer nanoparticles. In this work, stimuli-responsive magnetic particles composed of a magnetic poly(methyl methacrylate) core with a poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AA)) shell cross-linked with N,N -methylenebisacrylamide were prepared by miniemulsion polymerization. The particles were shown to have an average hydrodynamic diameter of 317 nm at 18 degrees C, which decreased to 277 nm at 41 degrees C due to the collapse of the thermo-responsive shell. The particles were superparamagnetic in behavior and exhibited a saturation magnetization of 12.6 emu/g. Subsequently, we evaluated the potential of these negatively charged stimuli-responsive magnetic particles in the purification of a monoclonal antibody from a diafiltered CHO cell culture supernatant by cation exchange. The adsorption of antibodies onto P(NIPAM-co-AA)-coated nanoparticles was highly selective and allowed for the recovery of approximately 94\% of the mAb. Different elution strategies were employed providing highly pure mAb fractions with host cell protein (HCP) removal greater than 98\%. By exploring the stimuli-responsive properties of the particles, shorter magnetic separation times were possible without significant differences in product yield and purity.
DOI http://dx.doi.org/10.1002/biot.201200329
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Publisher
Book Title
ISSN 1860-6768
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Conference Name
Bibtex ID ISI:000320031900012
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