Publication Type Journal Article
Title Quantitative enantioseparation of amino acids by comprehensive two-dimensional gas chromatography applied to non-terrestrial samples
Authors Iuliia Myrgorodska Cornelia Meinert Zita Martins Louis Le Sergeant D Hendecourt U. Meierhenrich
Groups
Journal JOURNAL OF CHROMATOGRAPHY A
Year 2016
Month February
Volume 1433
Number
Pages 131-136
Abstract This work presents an improved analytical procedure for the resolution and quantification of amino acid enantiomers by multidimensional gas chromatography. The procedure contains a derivatization step, by which amino acids were transformed into N(O,S)-ethoxycarbonylheptafluorobutyl esters. It was optimized for the resolution of non-proteinogenic amino acids in the matrix of complex non terrestrial samples. The procedure has proven to be highly sensitive and shows a wide linearity range with 0.005-3 pmol detection limits for quantitative determinations. The developed procedure was tested on a sample of the Murchison meteorite, for which obtained chromatograms show excellent peak resolution, minimal co-elution and peak overlap. We conclude that comprehensive two dimensional chromatography, in combination with the optimized derivatization method is a highly suitable technique for the analysis of samples with very limited quantities and containing potentially prebiotic molecules, such as interstellar ice analogs and meteorites. (C) 2016 Elsevier B.V. All rights reserved.
DOI http://dx.doi.org/10.1016/j.chroma.2016.01.014
ISBN
Publisher
Book Title
ISSN 0021-9673
EISSN 1873-3778
Conference Name
Bibtex ID ISI:000370086300014
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