Publication Type Journal Article
Title Reorganization of plasma membrane lipid domains during conidial germination
Authors Filipa C. Santos Andreia S. Fernandes Catarina A. C. Antunes Filipe P. Moreira Arnaldo Videira H. Susana Marinho R. F. M. de Almeida
Groups
Journal BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Year 2017
Month February
Volume 1862
Number 2
Pages 156-166
Abstract Neurospora crassa, a filamentous fungus, in the unicellular conidial stage has ideal features to study sphingolipid (SL)-enriched domains, which are implicated in fundamental cellular processes ranging from antifungal resistance to apoptosis. Several changes in lipid metabolism and in the membrane composition of N. crassa occur during spore germination. However, the biophysical impact of those changes is unknown. Thus, a biophysical study of N. crassa plasma membrane, particularly SL-enriched domains, and their dynamics along conidial germination is prompted. Two N. crassa strains, wild-type (WT) and slime, which is devoid of cell wall, were studied. Conidial growth of N. crassa WT from a dormancy state to an exponential phase was accompanied by membrane reorganization, namely an increase of membrane fluidity, occurring faster in a supplemented medium than in Vogel s minimal medium. Gel-like domains, likely enriched in SLs, were found in both N. crassa strains, but were particularly compact, rigid and abundant in the case of slime cells, even more than in budding yeast Saccharomyces cerevisiae. In N. crassa, our results suggest that the melting of SL-enriched domains occurs near growth temperature (30 degrees C) for WT, but at higher temperatures for slime. Regarding biophysical properties strongly affected by ergosterol, the plasma membrane of slime conidia lays in between those of N. crassa WT and S. cerevisiae cells. The differences in biophysical properties found in this work, and the relationships established between membrane lipid composition and dynamics, give new insights about the plasma membrane organization and structure of N. crassa strains during conidial growth. (C) 2016 Elsevier B.V. All rights reserved.
DOI http://dx.doi.org/10.1016/j.bbalip.2016.10.011
ISBN
Publisher
Book Title
ISSN 1388-1981
EISSN 0006-3002
Conference Name
Bibtex ID ISI:000392898100003
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