| Abstract |
Two novel bicapped Keggin polyoxidovanadates with organic cations, (C6H8N)(5 )[H4PV14O42]center dot 5H(2)O (1) and (C6H14N4)(2)(NH4)[H4PV14O42]center dot 11H(2)O (2), (PV14O426- = PV14, C6H7N = 3-picoline and C6H12N4 = methenamine) were synthesized. These compounds were isolated and characterized in the solid state and in solution by elemental analysis, powder X-ray diffraction, FTIR, UV-vis, V-51, P-31, C-13 and H-1 NMR, and fluorescence spectroscopy. Further confirmation of the PV14 structures was obtained by single-crystal X-ray diffraction studies of 1 and 2. The Hirshfeld surface analysis was performed to confirm that within the intermolecular interactions occurring in the two crystals, the O center dot center dot center dot H/H center dot center dot center dot O, O center dot center dot center dot O and H center dot center dot center dot H interactions dominate. The protonation and one-electron reduction of the PV14 moiety were also analysed by means of DFT calculations; besides confirming the protonation sites and correctly predicting the pK(a) values, the DFT results also indicate that molecular reduction is energetically more favourable in protonated PV14 anions. Upon the addition of PV14 anions to bovine serum albumin (BSA) up to a ratio of 1:1, the fluorescence decreased by 45\% for both 1 and 2, indicating that the interaction of vanadium-containing species with this protein takes place; log(K-SV) values of ca. 5.5 were obtained in both systems. Upon the addition of 1 or 2 to solutions of calf-thymus DNA (ctDNA), changes were observed in the UV-vis absorption and circular dichroism spectra. The significance of the changes observed is discussed considering the several V-containing species that form in the solution. |
