Publication Type Journal Article
Title Disclosing the potential of eleganolone for Parkinson s disease therapeutics: Neuroprotective and anti-inflammatory activities
Authors Joana Silva Celso Alves Susete Pinteus Patricia Susano Marco Simoes Miguel Guedes Alice Martins Stephanie Rehfeldt Helena Gaspar Marcia Goettert Amparo Alfonso Rui Pedrosa
Groups HC
Journal PHARMACOLOGICAL RESEARCH
Year 2021
Month
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Volume 168
Number
Pages
Abstract The treatment of Parkinson s disease (PD) has benefited from significant advances resulting from the increasing research efforts focused on new therapeutics. However, the current treatments for PD are mostly symptomatic, alleviating disease symptoms without reversing or retarding disease progression. Thus, it is critical to find new molecules that can result in more effective treatments. Within this framework, this study aims to evaluate the neuroprotective and anti-inflammatory effects of three compounds (eleganolone, eleganonal and fucosterol) isolated from the brown seaweed Bifurcaria bifurcata. In vitro neuroprotective effects were evaluated on a PD cellular model induced by the neurotoxin 6-hydroxydopamine (6-OHDA) on SH-SY5Y human cells, while lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages were used to evaluate the anti-inflammatory potential. Additionally, the underlying mechanisms of action were also investigated. Compounds were isolated by preparative chromatographic methods and their structural elucidation attained by NMR spectroscopy. Among the tested compounds, eleganolone (0.1-1 mu M; 24 h) reverted the neurotoxicity induced by 6-OHDA in about 20\%. The neuroprotective effects were mediated by mitochondrial protection, reduction of oxidative stress, inflammation and apoptosis, and inhibition of NF-kB pathway. The results suggest that eleganolone may provide advantages in the treatment of neurodegenerative conditions and, therefore, should be considered for future preclinical studies.
DOI http://dx.doi.org/10.1016/j.phrs.2021.105589
ISBN
Publisher
Book Title
ISSN 1043-6618
EISSN 1096-1186
Conference Name
Bibtex ID WOS:000655486900013
Observations
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